The Experimental Design And Its Budget

The experimental design and its budget

EXPERIMENTAL DESIGN

The facilities where experimentation animals are housed is called bioterio, and is the place dedicated to the care, parenting and maintenance of laboratory animals. And you have to meet requirements within it with the different areas.

The dirty zone that is costume, administrative and waste area and the clean area where animals are raised, in addition to laboratories and operating rooms.

To prevent the clean zone from being contaminated, these two areas must be separated by physical barriers, in addition to excellent cleaning, hygiene and disinfection of the areas. In addition, the areas must be regulated by ethical and legal norms;In addition to being supervised areas.

The animal’s microenvironment must always be regulated, since an enriched environment will improve stress and fear;As a consequence, it will present an easy handling of the animal and a rapid acclimatization;cages and bottle must be cleaned periodically. On the other hand, the macroambiente is fundamental, hygiene must be had in the accommodation room and control the environment such as temperature, humidity, ventilation and lighting.

The trained personnel must always be available to promote the welfare of the animal, for the moral and legal obligation but also to guarantee the reliable and repeatable results.

The main rodents can be classified into consanguineous strains that are characterized by genetic homogeneity, are in a registered genetic background and the non -consanguineous colonies, which can maximize genetic variation.

Of the most commonly used experimental models, the mouse is first, it is chosen to know them reactions of the organism in the face of poisoning, experimental infection, teratological reactions, is chosen among several things for its high reproductivity, easy maintenance.

Second, the rat is located, which is used for nutritional and endocrinological studies.

Another animal is the rabbit used for the innovation of anti -war, drugs, see toxicological effects and teratogenicity. Hamster is another animal that is used for reproduction and immunology studies.

There are currently a large number of animals used in experimental studies, depending on the needs of the researcher and the subject of the study project.

Other alternatives of the aforementioned animals are the prokaryotes, protists, fungi, invertebrates and lower vertebrates, but the most used alternative to date are cellular crops.

William Russell and Rex Burch, in 1959, publish a series of lines for the humanitarian use of laboratory animals, and define them as the three "RS" which means: reduce, replace and refine;This means that the use of animals in the least necessary number, but to obtain valid results. And if the alternative methods can be applied such as mathematical models, software simulators and cellular crops. The refinement refers to the standardization of parameters related to the genetic and warm definitions of the environment where animals grow, in addition to including all the methods that ensure the animal’s welfare with the minimination or elimination of pain before, during and after theexperimentation.

Financial aspects of research

1. Financial viability of the research project
2. Where and to whom to request a budget

Carrying out an investigation has a monetary value and this will depend on the topic to be investigated, these resources can obtain from competitive funds that can come from both public and private, national or international organizations. But for this it is necessary to make an assessment of the cost of the project, the more detailed it will be better since greater credibility will be contributed.

For a better organization you can make a table where all described what will be occupied from the initiation of the project to its term.

They can be divided into investment spending in which they include all the necessary infrastructure for the study, such as computer, photocopier, laboratory equipment with all the necessary instruments, tools and accessories. In addition, all bibliographic material such as magazines, books, etc. For this, sometimes you will need payment for access to articles or software for data analysis;or the case may need to register training courses.

And on the other hand, current spending is all that consumable expense that is needed to carry out the study, in addition to the work of professionals or technicians that will be required, another element in this section, are from transfers and food in academic events or update courses.

This budget must always be carried out and contributed before starting the research project, to ensure and confirm the viability of the project and leave it unfinished at the time of starting it.

It is important to take into account that government agencies and private companies are interested in finances in order to seek the most adapting for the investigation itself. CONACYT is a resource fund that supports the different investigations and should be proposed specifically and clearly all the necessary budget, in order to receive support, there are also hospitals that support the research and more when they are in the research center

Reference

• Hernández Sampieri, Roberto;et al. Investigation methodology. 6th. ed. McGraw-Hill. Mexico, d.F., 2014.
• Romero-Fernandez W, Batista-Castro Z, De Lucca M, Ruano A, García-Barceló M, Rivera-Cervantes M, García-Rodríguez J, Sánchez-Mateos S. 1, 2, 3 of experimentation with laboratory animals. Rev Peru Med Exp Health Public. 2016; 33 (2): 288-99. DOI: 10.17843/RPmesp.2016.332.2169
• Burdiles, p., Castro, m., & Simian, D. (2019). Planning and feasibility of a clinical research project. Las Condes Clinical Medical Magazine, 30 (1), 8-18.